Hyperpolarised 1H–13C Benchtop NMR Spectroscopy

Hyperpolarised organic phosphates as NMR reporters of compartmental pH.

Organic phosphate metabolites contain functional groups with pKa values near the physiologic pH range, yielding pH-dependent (13)C chemical shift changes of adjacent quaternary carbon sites. When formed in defined cellular compartments from exogenous hyperpolarised (13)C substrates, metabolites can thus yield localised pH values and correlations of organelle pH and catalytic activity.

Simple Methods for Measuring Total Oil Content by Benchtop NMR

Low-resolution time domain nuclear magnetic resonance (TD-NMR) is an internationally recognized and commercially important analytical tool for measuring the oil content in oilseeds and oilseed residues. It is commonly employed as a simultaneous determination of oil and moisture content, as in ISO 10565 or AOCS Ak 495. Because the method is very fast, compared with oven drying to determine moist...

Ex Vivo Spectroscopy - Linking the Benchtop to the Clinic

All the methods developed for studies on pure organic molecules (e.g. various kinds of 2D NMR, shifting the pH of the sample etc) can be used to improve peak resolution and assignment, and a wealth of information is available. In this lecture I shall not deal with the basic methodology of in vitro NMR, as this is covered in numerous basic textbooks [e.g. (2)]. Instead I shall concentrate on the...

NMR-Spectroscopy in Drug Analysis Quantitative NMR Spectroscopy

NMR Spectroscopy in Microfluidic Stripline NMR Chips

Microfluidic stripline NMR technology not only allows for NMR experiments to be performed on small sample volumes in the submicroliter range, but also experiments can easily be performed in continuous flow because of the stripline’s favorable geometry. In this study we demonstrate the possibility of dual-channel operation of a microfluidic stripline NMR setup showing oneand twodimensional H, C ...